Sunday, September 22, 2013

Happy World Rhino Day!!

Today is World Rhino Day!

It's the perfect time to stop and appreciate a magnificent species and to aid the conservation effort =]

My husband and I are both becoming more actively involved with Rhino Rescue Project, the group that we worked with in South Africa to perform the rhino horn infusions. We are both passionate about helping to save this species and are happy to help in any way that we can.

My husband did this amazing art piece for Rhino Rescue Project in honor of World Rhino Day. It's a continuation of his new word art series, and all of the words that make up the picture are related to rhino conservation.



I've been working on a new sewing project, where I am hand embroidering rhinos and am planning to make pillows and wall hangings out of them. I am hoping to be able to sell them and give a portion of the proceeds to Rhino Rescue Project. 



And since we were so successful with our bake sale fundraising last year, we are hoping to do our mobile bake sale again, with cupcakes and safari shaped cookies! Not only is baking a great way to procrastinate from studying, but then by selling them I don't have to feel guilty and obliged to eat everything I bake, and I can fundraise for rhinos at the same time! It's a win-win situation. =]

And hopefully one day we'll be able to go back to South Africa and work with Rhino Rescue Project again.



If you're interested in checking out the awesome work that Rhino Rescue Project is doing, here's the link to their facebook. Rhino Rescue Project


Tuesday, September 17, 2013

Just another day in the VMTH

It was another exciting equine med class today, as we got to try our hand at passing nasogastric tubes and performing abdominocentesis. All of this was complicated by the fact that the horses at the hospital have had tubes passed on them so many times that they are nearly impossible to work with. The bright side to that is just if you can get it done on them, you can get it done on any horse! We also had a little help... I gave our horse some xylazine IV to help calm her down. But even with that she still put up a fight.

I'd never passed a nasogastric tube before, though I'd seen it done. There are a lot of reasons to tube a horse, but the most common is probably colic. Every horses that is colicking gets a tube. So if you're going to be an equine practitioner, it's another skill you should probably keep in your back pocket, since nearly anything can make a horse colic.

The trick- and scary part, if you've never done it before- is actually passing the tube into the esophagus and not the trachea. It's surprising easy for the tube to go the wrong way, since it just naturally wants to go dorsal and not ventral. So once you get the horse to swallow and you think you have the tube in the right spot, you always have to stop and check. You should be able to feel two tubes in the horse's neck- one tube is the trachea, and the other tube is the nasogastric tube in the esophagus. When you suck in on the other end of the tube as well, there should be negative pressure (aka no air). Once you've confirmed you're in the trachea, you just keep sliding the tube down, blowing into it as you go to help expand the esophagus, until you stop getting negative pressure and you start getting positive pressure (aka air) when you suck in. Sometimes the change is very abrupt, and you end up inhaling stomach acid and pulling a bunch of stomach contents back up the tube. But at least you know you're in the right spot then! And sometimes exactly what you want is stomach contents, anyway.

For abdominocentesis, you have to feel for the xiphoid, or the end of the sternum. There are two muscles on the horse's belly that form a V right at the xiphoid. From there you go out a hand's width and a little to the right (to avoid the spleen that is sitting on the left), and just stick a needle in there. Nothing should happen with a normal horse, but with a sick horse you might get fluid coming out. Abdominocentesis isn't done a lot in equine medicine- you have to weigh the risks and benefits to a procedure, and our horse was a kicker- but it's also done for colics. If a horse is colicking and you get clear fluid, that's a better prognostic sign than if you get blood and a whole bunch of other nasty fluid, which might indicated a perforated bowel.





Getting the nasogastric tube into the ventral medial meatus is the most difficult part 


sliding the tube down into the esophagus


checking for negative pressure

Saturday, September 14, 2013

Polar Bear Reproduction

Now I'll be the first to admit that I'm a nerd for being so interested in this kind of topic, but I can't help myself. Anything to do with exotics, and especially if it's conservation related, and I am completely hooked.

Polar bears were listed as threatened by CITES in 2008, meaning that no more wild polar bears could be imported into US zoos. This posed a problem for the zoos, because polar bears do not breed well in captivity. Polar bears have a very high incidence of stillbirths and neonatal death, at least in a zoo setting. Overall, there is only about a 30-40% cub survival rate. Which is obviously not very good. And no matter what your views on zoos are (and I can admit too that my own views on zoos are mixed), I am at least happy that something has sparked an interest in people to look into polar bear reproduction and trying to help save the species.

Currently, there is no way to diagnose of confirm a pregnancy in a polar bear. You can't take a blood sample from a polar bear, and the urine samples are usually so contaminated that they're useless. These are both methods that are used to diagnose pregnancy in humans. We are also able to use ultrasound, something else which is useless in polar bears. You can go ahead and ultrasound a polar bear, but you're not going to see anything. The abdomen is just way too big, and their uterine horns are about the size of a pinky. I don't think that anyone has managed to find them or the uterus on an ultrasound yet. So that left researchers with fecal samples.

When working with fecal samples, researchers are looking for steroid metabolites, or specifically testosterone and progesterone. There does seem to be a way to use these hormones to distinguish between pregnancy and non-pregnancy, but not between pregnancy and pseudo-pregnancy, where the body pretty much acts like it's pregnant without actually being pregnant. Researchers are also trying to figure out where all of the pregnancy loss is occurring within the polar bear's reproductive cycle using these hormones. Knowing where the loss is occurring might lead to a way to have less pregnancy loss.

A polar bear's reproductive cycle is different from ours. For one thing, they are induced ovulators. So unlike humans, who ovulate about once a month regardless of what else is happening, polar bears will only ovulate if actually stimulated by a male to ovulate. They also have a period called diapause, which is a delayed implantation of the fetus in the uterus. Polar bears are seasonal breeders, with the peak breeding season between March and May. The embryo enters diapause throughout the summer, and doesn't implant in the uterus until around September or October. After implantation, there is about a 60 day gestation, with the baby being born around December. Research has found that testosterone spikes in females around ovulation and breeding, and progesterone spikes around the time of implantation and levels remain high until birth. These hormone spikes also occur in pseudopregnant females. A non-pregnant female pretty much just flatlines with her hormones throughout the year, with no spikes.

The next step is trying to use fecal proteins to distinguish between pregnancy and pseudopregnancy. Fecal samples were broken down and the proteins run with gel electrophoresis to separate them, and at the end researchers found that there are 5 proteins that were consistently more abundant in pregnant samples compared to non-pregnancy fecal samples. All of these fecal samples were retrospective, to be sure that it wasn't a case of pseudopregnancy. And 2 out of those 5 proteins have recognized roles in pregnancy- carboxypeptidase B and transthyretin. Transthyretin is produced by the placenta in early pregnancy. This may end up being a way to distinguish pseudopregnancy from an actual pregnancy.

Another method being looked at is actually the use of Sniffer dogs. Dogs are currently used for the detection of a lot of different things- anything from drug dogs to dogs that sniff out allergies and pull their owners away, to dogs who can distinguish people with colon cancer from those without it. So there was an idea to see if dogs could distinguish between pregnant and pseudopregnant females by feces. So far a little beagle named Elvis is distinguishing pregnant from pseudopregnant females with a 95% accuracy rate. He will be traveling around zoos this coming breeding season to try and predict which females are actually pregnant, and then we'll just have to wait and see which bears cub in December. Unfortunately though, there still is no way of telling if the bear is going through pseudopregnancy or if she lost the pregnancy.

Another thing being looked at is semen collection and artificial insemination. In the past, electroejaculation was used on male bears, which works well in other species but not at all in polar bears. It's believed that all of their blubber gets in the way. Now researchers are looking at chem-facilitated collection, where medetomidine is given to the bears, which causes semen to pool in the urethra. This can then be collected by catheterization. There is no urine contamination with this procedure, and it's fast- collection time is only 3-4 minutes. So far there has been really great success with this method. The semen can then be used to artificially inseminate a female, or be frozen for future use.

Researchers are also looking at which drugs to use to induce ovulation in females. On their first female they used ECG (equine chorionic gonadotropin) and porcine LH (luteinizing hormone), which is the standard in tigers. The female this procedure was used on did exhibit nesting behavior, but never produced cubs. No one knows if it was a pseudopregnancy or if she lost the pregnancy.

Recently at Sea World San Diego, one female was bred naturally and another was bred by artificial insemination. The bear was given 2 doses of ECG separated by 4 days and was given GnRH (gonadotropin releasing hormone) at the time of breeding. Sea World has their animals and staff much better trained at collecting samples- their dolphins are trained to pee in cups for urine samples and to present their tail fin for blood collection- so they are able to collect both urine and fecal samples. So far both females are exhibiting nesting behavior, so everyone is waiting to see if there are any cubs this December.

Overall, there has been some really exciting progress. No one had ever artificially inseminated a polar bear before, and even though it was unsuccessful researchers learned a lot about the female reproductive tract in females, when before we had known literally nothing. And with populations continuing to decline in the wild, every effort to help perpetuate the species is important.




Researchers from CREW ultrasounding a polar bear


Researchers at CREW ultrasounding a polar bear




Volpicelli Artwork

My husband has recently started doing "Word Art", where he uses lyrics and quotes to create portraits. It all started out just as an idea for an art project for school, and he had no idea if it would actually work or not. But it worked out better than he ever thought it could, and now it's taking off! The artwork is truly amazing. 

Here are some samples of what he has done so far.

Portrait of Malala Yousafzai, a woman known for her education and women's rights activism in Pakistan. She survived an assassination attempt by the Taliban in 2012 and has since been nominated for the 2013 Nobel Peace Prize. She was also the first recipient of Pakistan's first National Youth Peace Prize.

A commissioned portrait of Pope John Paul II


A portrait of Israel "IZ" Kamakawiwo'ole, a Hawaiian musician best known for his medley of "Somewhere Over the Rainbow/What a Wonderful World"


A portrait of Maxi Jazz, the lead singer of the British band Faithless. 


A portrait of Thich Nhat Hanh, a Vietnamese Zen Buddhist monk and anti-war and human rights activist. Martin Luther King Jr. nominated him for the Nobel Peace Prize in 1967.

An unfinished commissioned portrait of a friend's son. But it's looking really good so far, and it's one of the first ones done in partial color.


I love the way these word art pieces look! I'm trying to get my husband to do one of our lizard Sydney, but he's had so many requests that it looks like that one will have to wait. And these portraits helped secure him a job teaching a portrait art class at our local community center! It's always nice to know that hard work and persistence pay off =]

If you're interested in having a portrait done, the current prices are listed below. You can also contact him at volp91w@yahoo.com for more options. And if you want to see more of his artwork that isn't word art, his website is Master Portraits.


Sizing and Color Options

Wednesday, September 11, 2013

Solio

Before I went to South Africa I did a ton of research into the illegal ivory trade, and I still try to keep up to date on what's going on. But my interest had solely been focused on the elephant ivory trade, since I love elephants and hate the thought that they're being killed just for their teeth. But I never really focused that much on the rhino ivory trade, just because I didn't like rhinos as much I guess.

That all changed after two days working with rhinos in an active conservation effort. You can't spend all that time with rhinos and not appreciate them. I see rhinos in a completely different light now- their speed and power is unbelievable, and they are just magnificent creatures. And while in South Africa I learned some very alarming facts, including that rhinos are being poached at a rate of about 75 a week. 75 rhinos a week getting their face hacked off for what is essentially fingernails. 75 rhinos a week... that's nearly 4,000 rhinos a year. There are an estimated 20,000 rhinos left in South Africa, so at that rate the rhino could be extinct in as little as 5 years, if something isn't done to stop the trend.

I'm more interested in the ivory trade and conservation efforts than ever before. There seems to be promising signs- governments are taking more active roles, for example- but the black market ivory trade is like a chronic disease that is going to take a long time to fix, and it's going to take even more time for the populations to bounce back. There are a lot of people who are making small changes every day trying to help halt the disease spread, and that's what I want to be a part of.

I always admired the David Sheldrick Wildlife Trust and the work they do for conservation; I still foster two elephant orphans with them, who are both doing well. The trust is always looking for injured or abandoned orphans to take in and raise to be able to release back into the wild. They mainly take in elephant orphans, but occasionally they get called out for a rhino, too.

I believe that currently they have two rhino orphans, Solio and Maxwell. It takes a lot to care for a rhino orphan. This description of duties is directly from their website, http://www.sheldrickwildlifetrust.org.


"When rearing rhino orphans, it is important to:
  1. Replace the Mother Figure with several Keepers, so that the baby will follow more than one person and will not be reliant on just one, which will lead to problems should that person have time off.
  2. Protect the calf at night for the first three years, initially in a small stable, and later on in a spacious Stockade. Place an article of the Keeper's clothing in with the calf so that the familiar scent is there.
  3. Bottle feed a small calf four hourly throughout the day, but not at night, the last milk feed being at 6 p.m. When the calf is older and beginning to nibble on vegetation, anchor suitable cut greens within the stable at night and also offer a bowl of bran in which minerals are mixed as further supplementation.
  4. Walk a calf around dungpiles and urinals of the established wild community on a daily basis from dawn until dusk giving it time to investigate scent trails and contribute to the dungpiles. Allow it to eat the dung of the other animals (which all rhinos do) and which establishes the stomach bacteria needed.
  5. Take the calf to water and a mudwallow on a hot day so that it can drink and then lie and roll in the mud. Plaster the body in soft mud, which seals moisture in the skin, protects the animal from biting insects and sun. Mud is part of good skin care in all animals that do not have fur.
  6. Move a growing calf into a spacious Stockade at night once it has outgrown its Nursery quarters, because large animals become claustrophobic if confined too closely. Be sure to take its dung and establish a dungpile within the new quarters and also have a Keeper handy to calm the animal. Rhinos are creatures of habit and routine, and any break in the daily routine or happening is traumatic.
  7. When the calf is 3 or 4 years old, the Stockade doors can be left open at night, so that the calf can venture out and make physical contact with the other rhinos if it so wishes, but it is important that it can return to something familiar ("Home Base") whenever it feels the need to do so. By then, it will no longer be dependent on its Keepers, but will always respond to them. For instance, when Scud, as an adult, returned home injured, only her erstwhile Nursery Attendants could handle her.
N.B. Little rhinos are very playful, but must learn the boundaries of acceptable playfulness around humans. Therefore, discipline must be meted out in early infancy whilst a calf is small, initially with a sharp tone of voice and then the word "NO", and if necessary it can be reinforced by a prod on the nose from a small electric Cattle Prod powered by two torch cells. Rhinos are very easy to discipline, and are not normally disobedient in the same way as are elephants, so the electric prodder will only have to be used probably once or twice. However, whenever a rhino begins to gallop around playfully and becomes excited, it is a good idea to keep well out of the way!"

It takes a lot of patience and dedication to bring up an orphaned animal. If I had the time, space and money I would love to adopt them all and do something similar myself. Since I can't, I still like to do what I can. Which is why in addition to Quanza and Kanjoro, I now foster Solio. Solio was found standing over her mother, who had a shattered shoulder and was unable to stand or protect her calf. It's not clear if the injury was from a poaching attempt or not, but it would not be unheard of it it were. Some rhinos do manage to survive the immediate poaching attack, only to die of their wounds later. So Solio was brought to the orphanage when she was somewhere between 6 months old to a year old in 2010, and she's been there ever since. This month is her 3 year anniversary being at the orphanage, and she seems to be doing very well. She comes and goes from her stockade as she wishes, which is the beginning of the re-introduction process to the wild. She has her own space at the stockade, and she comes to eat and visit the other rhino, Maxwell, every couple of days or so. This July she was gone for a week before returning to the stockade, which the Keepers took as a good sign that she has been able to establish her own territory and that she is being accepted by the other wild rhinos. This is a great step towards her reintegration back into a wild life. 


When Solio first came to the orphanage, she was very feisty, and the keepers couldn't get near her to bottle feed her. That has changed as they earned her trust with their compassion and constant care.






Solio today


Tuesday, September 10, 2013

Equine Medicine


When I signed up for the advanced equine medicine elective I was expecting just a once a week lecture on something that involves horses. Maybe I'm biased, but I don't feel like we get a lot of information that relates to horses, it feels like a lot of small animal. But I'm sure the small animal lovers feel like we get too much large animal. We definitely get more equine than exotics, which is why I'm taking two exotic electives, but I'll save that for another post. 

Anyway, I expected the class to be just sitting in a classroom listening to, I don't know, common equine diseases or nutrition or something along those lines. Instead I was pleasantly surprised that for the majority of our class periods, we'll actually be spending them in the teaching hospital working on horses. And not just watching other people working on horses like all the intro to clinics classes, but actually working on them. Finally, some more hands on experience. 

Last week it was lameness exams, and this week it was jugular catheters and urinary bladder catheters. Lameness exams are extremely routine in equine practice, so even if you're just interested in horses you probably know what a lameness exam entails. Horses are always going lame, which just means they're not walking right for one reason or another. And if you're doing it in Oklahoma, you definitely want to be the one watching the horse and not the one jogging it in the 100 degree heat. But basically all a lameness exam entails is jogging the horse and trying to see which leg it's lame on. There are a couple tricks to help- the horses head will bob up on the lame leg if it's in the front, and the hip raises on the lame leg in the back. Sometimes the lameness is subtle, so you can use a flexion test to try and exacerbate the lameness and make it more obvious. For the flexion test, you have to isolate each joint and jog the horse after each joint flexion, otherwise it wouldn't help you narrow down which part of the leg was the cause of the lameness. If the lameness is coming from the joint you’ve just flexed, they will be noticeably more lame on the next job.

If the lameness is in the front legs, for the majority of the time the problem is going to be in the feet. Even if it looks like it’s coming from somewhere else, say, the shoulder, a lot of the time the horse is just trying to compensate. If the lameness is in the hind legs, and lot of the time the problem is in the hock joint (the equivalent of our ankle). If there is lameness in both the front and the hind limbs, look at which sides of the body they are on, and that can give you a clue as to which is the primary lameness and which one is compensatory. If the limbs are lame on the same side of the body, it’s more likely that the primary lameness is in the front limb. If the horse is lame on the diagonal, such as the right front and the left hind, it’s more likely that the primary lameness is in the hind limb. These don’t hold true for every case, but it’s always a good starting point.  And you just have to train your eyes to watch the entire horse as it moves to pick up the clues, as not all lameness are obvious. Most of the time a horse is completely sound while walking, and you might only pick it up intermittently while jogging/trotting.

That’s pretty standard procedure for horse care. If you own a horse, at some point it will be lame. So I was pretty excited to do a urinary bladder and jugular catheter, because that I don’t get to do every day. Not that I do a lameness exam every day either, but when I was shadowing vets I did quite a few of them, and I was always the one who got to jog the horse. I got a lot of exercise those summers.


Although something new that the school was doing that I like was using a sensor system during the lameness exam. The horse gets a little head mask with a sensor, a sensor around the ankle, and a sensor on top of the hips. Then when you jog the horse, it sends the information to a computer that tells you where the horse is lame after monitoring its movements. I’m not sure how well the system works, since this was the first time I’ve seen it used. But I do know one downfall is the sensors can’t tell a pacer from a trotter, which would reverse your lame leg if it was in the hind limbs, and it can’t tell if your jogging straight or in a curve, which would affect your lameness score. But those are pretty minor details.


To insert a urinary bladder catheter you have to use sterile technique, which means just cleaning off the vulva before sticking your catheter in. It’s always fun when, right after you’ve finished cleaning, your horse decides it has to go to the bathroom. Then you get to start all over. When you’re ready to go, you have to slide the catheter into the vulva and try to find the little slot leading to the urethra. It feels like a little knob, and if you poke it your finger should slide right in. I found it was easiest to stick my middle finger in, and then slide the catheter in under my finger a bit, before removing the finger and sliding the catheter in more. You don’t really have to go that deep at all- as opposed to all of the artificial inseminations I did where I was literally up to my shoulder in the mare, placing a urinary catheter really only puts you in up to your wrist. That’s a good check to make sure you’re not going to the wrong place. And then you just keep sliding the catheter in until urine comes out. You should pay attention to where you’re holding the other end of the catheter, or you may end up spraying urine all over your shoes or shooting it at the person standing next to you, which I almost did by accident.

For the jugular catheter, it’s the same theory as in dogs and cats except you get a bigger vein to hit. Unfortunately, that vein likes to fill very slowly in some horses, making it more difficult to see. You always want to insert the catheter in the jugular groove, where there is muscle underneath the jugular vein that helps protect the carotid artery. The very last thing you ever want to do is hit the carotid when you’re aiming for the jugular.

Horse skin is pretty thick, so you have to use a good jab to get the needle going in. Then once you get the flash (blood coming out of your catheter), you slowly advance the plastic, flimsy catheter off of the metal needle further into the jugular vein. And that’s all there is to it. Be prepared to get blood all over your hands though when doing this, just because a horse is pumping a lot of blood, and you don’t have anything to stop if from coming out when you don’t have anything hooked up to your catheter.

I love getting hands on experiences like this. First, because it’s always so much more interesting that sitting in a classroom just listening to the theory on how to do something. But it also stays in your brain better once you’ve actually done it. Because you could tell me what it feels like to insert a urinary catheter as many times as you want and how to do it, but until I can feel that little bump and slot for myself, I’m still going to struggle the first time I do it. So basically, I feel like I’m just eliminating a step and getting further ahead by doing so.

I can’t wait to see what else we’ll get the chance to do this semester.